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KMID : 0378019730160060063
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New Medical Journal
1973 Volume.16 No. 6 p.63 ~ p.69
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Studies on the Erythrocyte G-6-PD Measurement and Values in Korean
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Abstract
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1. On the studies of hemolyzing solution we were obtained such optimum concentration of enzyme G-6-PD activity as followings: 2.7mM of EDTA, 5mM beta-mecrcaptoethanol, and 10 pM NADP.
2. Many different experimental conditions with relation to the measurement of enzyme activity were studied and obtained as following results:
a) The production rate of NADPH was constant during first 30 minutes after adding G-6-P into the reaction mixture.
b) Enzyme concentration, 1 to 6 volumes of usual concentration, obeyed by Beer´s law.
c) Influence of temperature was so significant that we used to a temperature of 25°C throughout the experimeatal procedures.
d) The reaction mixture except G-6-P was stable on 8 days or more in deep freezer (-45°C) and about 5 days in refrigerator (5°C).
e) The samples to be examined were stable on 3 hours at room temperature in case of intact whole blood and on 1 hour or less in case of hemolysates.
3. Erythrocyte G-6-PD values in Korean was such as follows:
The distribution of 294 males was 7.82±4.77, and 286 females 7.24±4.23 IU per gm of Hb. Age distributions of both groups, male and female, were 1 year or less to 75 years old which contained 5-10 healthy individuals in each age groups.
4. There was a groups strongly suggested such as G-6-PD Hektoen those enzymic value is shown about three times of mean value on the distribution curve.
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